CHEN HAI-BAO, Professor
Department of Bio-organic Chemistry
Date of Birth: 1938. 2
Education:
Graduated from Chemistry Department of Perkin University (1962)
from postgraduate study (tutor: Prof. Wang, Yu) in SIOC (1966)
As a visiting scholar in Khorana's Lab of M.I.T., USA (1984 - 1986)
Jion in SIOC: 1966
Tel: 86-21-64163300(ext.1429 or 1531)
E-mail: [email protected];
or E-mail: [email protected]
Research Interests:
Total Synthesize Derivatives of Dihydrostreptosonic monoacid g-lactone (1966)
Studies on Preparing Reversible Absorbent of CO2 for Nuclear Submarine (1968)
Nucleic Acids Chemistry:
Total Synthesis of Yeast Alanine tRNA (1970-1982)
Genetic Engineering and Protein Engineering:
Protein Engineering on Bovine Rhodopsin (1984-1986)
Genetic Engineering on Trichosanthin (1988-1994)
Genetic Engineering and Protein Engineering on higher Plant RubisCO (1988-)
Metabolic Engineering on CO2 Fixation (1995-)
Chemical Biology:
Screening the target protein with encoding gene of active organic compound
(2000-)
Achivement Award:
First Class National Natural Science Prize (1987)
Selected Publication:
1. Total synthesis of yeast alanine tRNA, Scientia Sinica [ B series] (1983)
2. Cysteine residue 110 and 187 are essential for the formation of correct
structure in bovine rhodopsin, Proc. Natl. Acad. Sci. USA.(1988)
3. A new method for the synthesis of a structural gene, Nucl. Acids Res.
(1990).
4. Design, total synthesis and highly expression in E.coli of a structural
gene for trichosanthin, Progress in Natural Science (in Chinese, 1995)
5. Studies on genetic engineering of rice Rubisco, Photosynthesis: from
light to biosphere (1995).
6. Overexpression in Escherichia coli and Characterization of the
Chloroplast Triosephosphate Isomerase from Spinach. Protein Expression
and Purification (1999)
7. Properties of Hybrid Enzymes between Synechococcus Large Subunits and Higher
Plant Small Subunits of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase in
Escherichia coli, Archives of Biochemistry and Biophysics (2001)
8. Two-cistron system overexpression of chloroplast glyceraldehyde-3-phosphate
dehydrogenase subunit B and B-derived derivatives from spinach in Escherichia
coli, Protein Expression and Purification (2001)
9. Co-expression of triosephophate isomerase, fructose-1,6-bis-phosphate
aldolase and fructose-1,6-bisphosphatase in E. coli, Acta Biochimica et
Biophysica Sinica (2001) (in Chinese).
Publications:
1. Huang Ying and Chen, H.-B., Active Recombinant Alpha Scorpion Neurotoxin
rBmKaIT1--Gene Synthesis, Expression in Escherichia coli, Purification and
Characterization, in the Proceedings of 7th Chinese (international) peptide
symposium July, 2002 Dalian, China. To be published.
2. Wang, Y.-L., Zhou, J.-H., Wang, Y.-F., Bao, J.-Sh., and Chen, H.-B.,
Properties of Hybrid Enzymes between Synechococcus Large Subunits and Higher
Plant Small Subunits of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase in
Escherichia coli, Archives of Biochemistry and Biophysics (2001) 396, 35-42.
3. Jiang, H., Ma, J.-B., Zhu, X.-M., Hui, Y.-Zh., Wu, H.-M. and Chen, H.-B.,
Synthesis of biotinylated cyclosporin A and studies on its interaction with
human cyclophilin A, Acta Chimica Sinica (2001) 59(10), 1745-1750 (in Chinese).
4. Tang, G.-L., Wang, Y.-F., Bao, J.-Sh. and Chen,H.-B., Two-cistron system
overexpression of chloroplast glyceraldehyde-3-phosphate dehydrogenase subunit
B and B-derived derivatives from spinach in Escherichia coli, Protein
Expression and Purification (2001), 22(1), 31-37.
5. Tang, G.-L., Yang, Ch.-S., Wang, Y.-F., Bao, J.-Sh., Chen,H.-B., Shi, D.-J.
& Liu, F.-L., Co-expression of triosephophate isomerase,
fructose-1,6-bisphosphate aldolase and fructose-1,6-bisphosphatase in E. coli,
Acta Biochimica et Biophysica Sinica (2001), 33 (1), 131-136 (in Chinese).
生物化学与生物物理学报(2001),33(1), 131-136。
6. Tang, G.-L., Ma, J.-B., Wu, H.-M. & Chen,H.-B., Novel technologies for
study on the interaction between active small molecules and biological targets,Kexue
Tongbao (2000), 45, 1345-1357 (in Chinese).
科学通报(2000),45,1345~1357。
7. Tang, G.-L. & Chen,H.-B., Recent development in metabolic engineering,YouJi
HuaXue (2000) 20, 634-640 (in Chinese)
有机化学(2000),20,634-640。
8. 陈海宝 聂慧玲,天花粉蛋白结构基因的DNA顺序测定,《天花粉蛋白》第二版,汪猷 金善炜编,科学出版社,北京,pp.211~217,2000年6月.
9. 陈海宝 聂慧玲,基因工程,《天花粉蛋白》第二版,汪猷 金善炜编,科学出版社,北京,pp.218~224,2000年6月.
10. Tang, G.-L., Wang, Y.-F., Bao, J.-Sh. and Chen,H.-B., Overexpression in
Escherichia coli and Characterization of the Chloroplast Fructose-1, 6-
bisphosphatase from Wheat, Protein Expression and Purification (2000) 19,
411-418.
11. Zhou, J.-H. & Chen, H.-B., A facile DNA shuffling protocol, progress in
Biochemistry & Biophysics (2000),27, 655-657(in Chinese)。
周佳海,陈海宝,生物化学与生物物理进展 (2000),27, 655-657.
12. Wu, Y.-L., Chen, H.-B., Jiang, K., Li Y., Shan, F., Wang, D.-Y., Wang,
Y.-F., Wu, W-M., Wu, Y.-K., Yao, Z.-J., Yue, Z.-Y. And Zhou, C.-M., Interaction
of biomolecules with qinghaosu(artemisinin) and its derivatives in the presence
od ferrous ion ? an exploration of antimalarial mechanism, Pure Appl. Chem.
(1999) 71, 1139-1142.
13. Tang, G.-L., Wang, Y.-F., Bao, J.-Sh. and Chen,H.-B., Overexpression in
Escherichia coli and Characterization of the Chloroplast Triosephosphate
Isomerase from Spinach, Protein Expression and Purification (1999) 16, 432-439.
14. Chen, H.-B .& Yang Ch.-S., Cloning and high expression in E.coli of a
chimeric gene coding for rice fructose-1,6-bisphosphate aldolase, Acta
Biochimica et Biophysica Sinica, (1997) 29, 613-616 (in Chinese).
生物化学与生物物理学报 (1997)29, 613-616.
15. Wu, W.-M., Yao, Z.-J., Wu, Y.-L., Jiang, K., Wang, Y.-F., Chen, H.-B.,
Shan, F., Li, Y., Ferrous Ion Induced Cleavage of the Peroxy Bond in Qinghaosu
and its Derivatives and the DNA damage Associated with this Process, Chem.
Commun. (1996), 2213-2214.
16. Wang, G.-A., Chen, H.-B., Wang, Y., Studies on genetic engineering of rice
Rubisco, in "Photosynthesis: from lightto biosphere" Vol V,, Mathis,
P. Ed., Kluwer Academic Publishers, Netherlands, pp. 91-94, 1995.
17. 陈海宝 夏 懿 荆军平 蒋 昆 鲍建绍,天花粉蛋白结构基因的重新设计、全合成及其在大肠杆菌中的高表达,自然科学进展- 国家重点实验室通讯(1995)第5卷第1期,
114-118页。.
18. Chen, H.-B., Xia, Y., Jing, J.-P., Jiang, K., Bao, J.-Sh., Chemical
Synthesis of a Structural Gene Coding for Trichosanthin, Chinese J. Chem.
(1995) 13:4, 349-357.
19. Chen, H.-B., Weng, J.-M., Bao, J.-Sh., Chemical Synthesis of a Structural
Gene Coding for Small Subunit of Ribulose-1,5-bisphosphate caboxylase /
oxygenase from Tobacco, Chinese J. Chem. (1994) 12, 355-364.
20. 陈海宝,1993年诺贝尔化学奖简介 ? 简评化学研究在生物学科发展中的贡献和展望,HuaXue TongBao (1994) 8: 38-40.
21. 陈海宝,开创研究遗传物质的新方法,Science pictorial (1994) No.1, 8-9.
22. Chen, H.-B., Wang, G.-A., Synthesis and Expression of a Gene for Mature
Small subunit of Rice Rubisco, Curr. Plant Sci. Biotecnol. Agric. (1993) 15
(Biotechnology in Agriculture), 160-164.
23. Chen, H.-B., Total Synthesis and Expression in E. coli of Three Redesigned
Genes for the Mature Small Subunit ( rbcS ) of Rubisco from Tobacco, Wheat and
Rice, YouJi HuaXue ( Chinese J. of Organic Chemistry) (1993) 13, 309-313.
24. Chen, H.-B., Li, J.-W., Yang, J., Expression in E. coli of a Chemically
Synthesized Gene for the Mature rbcS from Tobacco, in "Research in
Photosynthesis", Vol. III, Ed. by Murata, N., Kluwer Academic Publishers,
Netherlands, pp. 645-648, 1992.
25. 陈海宝 刘国诠, 化学与未来的生物学, 王亚辉 吴志纯主编的"走向21世纪的生物学 ? 未来生物学(1991-2020)年预测" 中国科学院科技政策局,
华夏出版社出版, pp168-182, 1992.
26. Chen, H.-B., Weng, J.-M., Bao, J.-Sh., Total Synthesis of a Gene for the
Mature Small Subnuit of RubisCO from Tobacco, in "Current Research on
Photosynthesis", Vol. III, Ed. by Baltscheffsky, M., Kluwer Academic
Publishers, Netherlands, pp. 347-351, 1990.
27. Chen, H.-B., Weng, J.-M., Jiang, K., Bao, J.-Sh., A New Method for the
Synthesis of a Structural Gene, Nucleic Acids Research (1990) 18, 871-878.
28. 陈海宝,核酸的有机化学研究在分子生物学发展中的作用,YouJi HuaXue (1990) 10, 298-327.
29. Braiman, M., Bubis, J., Chen, H.-B., Doi, T., Flitsch, S.L., Franke, R.R.,
Gilles-Gonzalez, M.A., Graham, R.M., Karnik, S.S., Khorana, H.G., Knox, B.E.,
Krebs, M.P., Marti, T., Mogi, T., Nakayama, T., Oprian, D.D., Puckett, K.L.,
Sakmar, T.P., Stern, L.J., Subramaniam, S. & Thompson, D.A. Studies on
Light Transduction by Bacteriorhodopsin and Rhodopsin, Cold Spring Harbor Symp.
Quant. Biol. (1988) 53, 355-364.
30. Karnik, S.S., Sakmar, T.P., Chen, H.-B., Khorana, H.G., Cysteines 110 and
187 are Essential for the Formatiion of Correct Structure in Bovine Rhodopsin,
Proc. Natl. Acad. Sci. USA (1988) 85, 8459-8463.
31. Karnik, S.S., Sakmar, T.P., Franke, R.R., Knox, B.E., Oprian, D.D., Chen,
H.-B., Khorana, H. G. Site-specific Mutagenesis of Bovine Rhodopsin, in
"Molecular Biology of the Eye: Genes, Vision and Ocular Disease",
UCLA Symposium on Molecular and Cellular Biology, New Series, Vol. 88, Ed. by
Pialigorsky, J., Shinohara, T. , and Zelenka, P. , Alan R. Liss, Inc., New
York, NY., pp. , 23-44,1988.
32. Oprian, D.D., Sakmar, T.P., Nakayama, T., Chen, H.-B., Knox, B.E., Karnik,
S.S., Franke, R. R., and Khorana, H. G. Molecule Biological Studies of Visual
Pigment, Rhodopsin, in Discussions in Neurosciences FESN " Vol 4 (3), Ed.
by Hudspeth, A.J., MacLeish, P.R., Margolis, F.L &Wiesel, T.N.,Geneva,
Switzerland, pp. 20-29, 1987.
33. Chen, H.-B., Zhuang, J.-H., Zheng, Y.-D.& Hu, M.-H., Synthesis of the
5'-Half Molecule Pentatricontanucleotide (35p) of Yeast Alanine Transfer
Ribonucleic Acid, Kexue Tongbao, (1984) 29, 109-115.
34. Chen, H.-B., Xu, J.-F., Bao, J.-Sh., Zhuang, J.-H., Guo, R.-H., Shi,
C.-N.,Chen, F.-X., Zhang, G.-J., Fong, X.-L., Jiang, P., Ding, X.-H.,Synthesis
of the 3'-Termina Nonadecanucleotide (58-76) of Yeas Alanine tRNA, Acta Chimica
Sinica, Engl. Ed., (1983) 1, 212-229.
35. Wang, D.-B., Zheng, K.-Q., Qiu, M.-S., Liang, Z.-H., Wu, R.-L., Chen,
C.-Q.,Wang, E.-B., Zhu, Y.-S., Shen, Q.-X., Yu, Y.-H., Wang, Y., Chen, H.-B.,
Yang, Z.-W., Lu, Y.-H., Chen, Z., Wang, G.-H., & Hu, M.-H., Total Synthesis
of Yeast Alanine Transfer Ribonucleic Acid, Scientia sinica (series B,1983) 26,
464-481.
36. Wang, D.-B., Qiu, M.-S., Liang, Z.-H., Zheng, K.-Q., Wu, R.-L., Wang,
C.-Y., Liu, X.-Y., Zheng, H.-D., Bao, Y.-D., Zhu, X.-L., Guo, L.-H., Chjen,
Y.-D., Huang, J.-J., Zhou, F.-Y., Chen, H.-B., Xu, B.-Zh., Zhang, Q.-J., Hua,
L., & Hu, M.-H., Synthesis of 3'-Half Molecule of Yeast Alanine tRNA,
Scientia sinica (series B,1983) 26, 482-494.
37. Wang, D.-B., Wu, R.-L., Zheng, K.-Q., Yu, D.-W., Tang, J.-Y., Qi, G.-R.,
Chen, C.-Q., Zhu, Y.-S., Xie, H.-Y., Wang, Y.-K., Nie, H.-L., Chen, H.-B.,
Huang, J.-H., Chen, D.-H., Zhuang, J.-H., Wang, G.-H., Chen, S., & Hu,
M.-H., Synthesis of 5'-Half Molecule of Yeast Alanine tRNA, Scientia sinica
(series B,1983) 26, 495-503.
38. Shanghai Institute of Biochemistry, Academia Sinica; Shanghai Institute of
Cell Biology, Academia Sinica; Shanghai Institute of Organic Chemistry,
Academia Sinica; Institute of Biophysics, Academia Sinica; Department of
Biology, Peking University and Shanghai No.2 Reagent Factory, Total Synthesis
of Yeast Alanine Transfer Ribonucleic Acid, Kexue Tongbao(1982) 27, 261-269.
39. Collaboration Group of Nucleic Acid Synthesis, Studies on the Synthesis of
Polynucleotides V. ---Synthesis of a Nonadecaribonucleotide Fragment (58-76) of
the Yeast Alanine tRNA in "Nucleic Acids and Proteins", The 1979
Shanghai Proceedings of PRC-FRG Join Symposium on Nucleic Acids and Proteins,
Ed. by Z.-W. Shen, pp. 272-281, Science Press, Beijing (van Nostrand Reinhold
Company, New York/Toronto/London), 1980.
40. Collaboration Group for Nucleic Acid Synthesis, Synthesis of 3'-Half Molecule
of Yeast Alanine tRNA, Kexue Tongbao, (1980) 25, 525-527.
41. Nucleic Acid Group, Shanghai Institute of Organic Chemistry, Academia
Sinica, Studies on Nucleic Acid Chemistry III. Synthesis of ApUpUpC, CpGpGpA
and ApUpUpCp of TUC Arm of Yeast Alanine tRNA, Acta Chimica Sinica (1980) 38,
1-17(in Chinese).
42. Nucleic Acid Group, Shanghai Institute of Organic Chemistry, Academia
Sinica, Studies on Nucleic Acid Chemistry I. Crystalline 5'-O-Trityl and
5'-O-Mono-p-Methoxytrityl Ribonucleotides, Acta Chimica Sinica (1979) 37,
299-304 (in Chinese).
43. Nucleic Acid Group, Shanghai Institute of Organic Chemistry, Academia
Sinica, Studies on Nucleic Acid Chemistry II. The Application of
N-Acylimidazoles in the Acyla- tions of the Ribonucleotides, Acta Chimica Sinica
(1979) 37, 305-314(in Chinese).
44. Collaboration Group of Nucleic Acid Synthesis, Studies on the Synthesis of
Polynucleotides --- Synthesis of a Dodecaribonucleoside Undeca-phosphate and
Hexadecaribonucleoside Pentadecaphosphate, Scientia Sinica, (1978) 21, 687-697.
45. 陈海宝, 有机化学方法合成寡聚核糖核苷酸的概况,Youji Huaxue (1976) No 1: 47-60.